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BIO-TECHNOLOGY 3rd Sem PDF Notes Free Download Kashmir University

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Q: -How does a centrifugation work?
A: -The centrifuge works using the sedimentation principle, where the centripetal acceleration causes denser substances and particles to move outward in the radial direction. At the same time, objects that are less dense are displaced and move to the center.
Q: -Why do you centrifuge blood?
A: -This is typically done by centrifuging the blood. The resulting components are: a clear solution of blood plasma in the upper phase (which can be separated into its own fractions, see Blood plasma fractionation), ... erythrocytes (red blood cells) at the bottom of the centrifuge tube.
Q: -Why we do centrifuge?
A: -The centrifuge is an instrument used in nearly every research lab across the globe. ... By spinning laboratory samples at very high speeds, the components of a given mixture are subjected to centrifugal force, which causes more dense particles to migrate away from the axis of rotation and lighter ones to move toward it.
Q: -What is the process of centrifugation?
A: -Centrifugation is a separation process which uses the action of centrifugal force to promote accelerated settling of particles in a solid-liquid mixture. Two distinct major phases are formed in the vessel during centrifugation: The sediment.
Q: -What is the application of centrifugation?
A: -Centrifugation is a process which involves the application of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings. ... The larger the size and the larger the density of the particles, the faster they separate from the mixture.
Q: -What is a gel electrophoresis?
A: -Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an electrical field through a gel that contains small pores.
Q:-What is coefficient of sedimentation?
A: -The sedimentation coefficient s of a particle is used to characterize its behaviour in sedimentation processes, notably centrifugation. It is defined as the ratio of a particle's sedimentation velocity to the acceleration that is applied to it (causing the sedimentation).
Q: -What is the meaning of ribosome?
A: -Bacteria and archaebacteria have smaller ribosomes, termed 70S ribosomes, which are composed of a small 30S subunit and large 50S subunit. The "S" stands for Svedberg’s, a unit used to measure how fast molecules move in a centrifuge.

Q: -What is the basic principle of sedimentation?

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